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2光子顕微鏡を用いた閉鎖頭蓋外傷性脳損傷マウスおよび頭蓋窓における蛍光タンパク質発現の生体内イメージング
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Neuroscience
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JoVE Journal Neuroscience
Intravital Imaging of Fluorescent Protein Expression in Mice with a Closed-Skull Traumatic Brain Injury and Cranial Window Using a Two-Photon Microscope
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2光子顕微鏡を用いた閉鎖頭蓋外傷性脳損傷マウスおよび頭蓋窓における蛍光タンパク質発現の生体内イメージング

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867 Views

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08:25 min

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April 21, 2023

DOI:

10.3791/64701-v

DOI:
10.3791/64701-v

•

08:25 min
April 21, 2023

•

3 Views
, , , ,
1Department of Neurology,University of Massachusetts Chan Medical School, 2Department of Neurosurgery,The First Affiliated Hospital of Chongqing Medical University, 3Department of Neurobiology,University of Massachusetts Chan Medical School

Summary

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この研究は、マウスへの反復性外傷性脳損傷の送達と、2光子顕微鏡を使用したニューロン発現EGFPのその後の生体内イメージングのための頭蓋窓の同時移植を示しています。

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Cite this Article

Zhong, J., Gunner, G., Henninger, N., Schafer, D. P., Bosco, D. A. Intravital Imaging of Fluorescent Protein Expression in Mice with a Closed-Skull Traumatic Brain Injury and Cranial Window Using a Two-Photon Microscope. J. Vis. Exp. (194), e64701, doi:10.3791/64701 (2023).

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