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JoVE Journal Neuroscience
Intravital Imaging of Fluorescent Protein Expression in Mice with a Closed-Skull Traumatic Brain Injury and Cranial Window Using a Two-Photon Microscope
 

Intravital Imaging of Fluorescent Protein Expression in Mice with a Closed-Skull Traumatic Brain Injury and Cranial Window Using a Two-Photon Microscope

Article DOI: 10.3791/64701-v • 08:25 min • April 21st, 2023
April 21st, 2023 •
1,2, 3, 1, 3, 1
1Department of Neurology, University of Massachusetts Chan Medical School, 2Department of Neurosurgery, The First Affiliated Hospital of Chongqing Medical University, 3Department of Neurobiology, University of Massachusetts Chan Medical School

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Automatic Translation

This study demonstrates delivery of a repetitive traumatic brain injury to mice and simultaneous implantation of a cranial window for subsequent intravital imaging of a neuron-expressed EGFP using two-photon microscopy.

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Intravital Imaging Fluorescent Protein Expression Mice Closed-skull Traumatic Brain Injury Cranial Window Two-photon Microscope Protein Of Interest Exogenous Stimuli Intracranial Injection Adeno-associated Virus (AAV) Enhanced Green Fluorescent Protein (EGFP) Neuronal Specific Promoter Weight Drop Device Metal Headpost Glass Cranial Window Expression And Cellular Localization Longitudinal Visualization
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