/
/
Intravital Imaging of Fluorescent Protein Expression in Mice with a Closed-Skull Traumatic Brain Injury and Cranial Window Using a Two-Photon Microscope
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich. Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
Intravital Imaging of Fluorescent Protein Expression in Mice with a Closed-Skull Traumatic Brain Injury and Cranial Window Using a Two-Photon Microscope
853 Views
•
08:25 min
•
April 21, 2023
DOI:
Summary
Automatically generated
This study demonstrates delivery of a repetitive traumatic brain injury to mice and simultaneous implantation of a cranial window for subsequent intravital imaging of a neuron-expressed EGFP using two-photon microscopy.
Explore More Videos
Intravital ImagingFluorescent ProteinTraumatic Brain InjuryCranial WindowTwo-photon MicroscopyNeuronal ResponseMechanical StressGene Of InterestVirus PromoterCell TypesOphthalmic OintmentMidline IncisionPeriosteumStereotaxic FrameTBI Impact SiteImpactor TipDental CementLateral MusclesCranial Window Implantation
Read Article
Cite this Article
Zhong, J., Gunner, G., Henninger, N., Schafer, D. P., Bosco, D. A. Intravital Imaging of Fluorescent Protein Expression in Mice with a Closed-Skull Traumatic Brain Injury and Cranial Window Using a Two-Photon Microscope. J. Vis. Exp. (194), e64701, doi:10.3791/64701 (2023).
Copy