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Generazione di cellule Natural Killer da cellule staminali umane espanse
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Generation of Natural Killer Cells from Human Expanded Potential Stem Cells
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Generazione di cellule Natural Killer da cellule staminali umane espanse

italiano

Automatically Generated

2,461 Views

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06:53 min

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January 13, 2023

DOI:

10.3791/64608-v

DOI:
10.3791/64608-v

•

06:53 min
January 13, 2023

•

11 Views
, , , ,
1Major in Molecular Biology and Biotechnology, School of Biological Sciences,The University of Hong Kong, 2School of Biomedical Sciences,The University of Hong Kong, 3Centre for Translational Stem Cell Biology

Summary

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Il presente protocollo mostra come differenziare le cellule CD3−/CD45+CD56+ con citotossicità lieve dalle cellule staminali umane a potenziale espanso (hEPSC) sia in condizioni di coltura 3D che 2D. Ciò consente la convalida fenotipica di routine senza la distruzione del complesso microambiente.

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Cite this Article

Yu Ching, P., Wang, C., Hang, S., Liu, P., Sugimura, R. Generation of Natural Killer Cells from Human Expanded Potential Stem Cells. J. Vis. Exp. (191), e64608, doi:10.3791/64608 (2023).

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