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Generation of Natural Killer Cells from Human Expanded Potential Stem Cells
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Gelişim Biyolojisi
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JoVE Journal Gelişim Biyolojisi
Generation of Natural Killer Cells from Human Expanded Potential Stem Cells

Generation of Natural Killer Cells from Human Expanded Potential Stem Cells

2,455 Views

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06:53 min

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January 13, 2023

DOI:

10.3791/64608-v

DOI:
10.3791/64608-v

•

06:53 min
January 13, 2023

•

11 Views
, , , ,
1Major in Molecular Biology and Biotechnology, School of Biological Sciences,The University of Hong Kong, 2School of Biomedical Sciences,The University of Hong Kong, 3Centre for Translational Stem Cell Biology

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The present protocol shows how to differentiate CD3−/CD45+CD56+ cells with mild cytotoxicity from human expanded potential stem cells (hEPSCs) under both 3D and 2D culture conditions. This allows for routine phenotypical validation without the destruction of the complex microenvironment.

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Yu Ching, P., Wang, C., Hang, S., Liu, P., Sugimura, R. Generation of Natural Killer Cells from Human Expanded Potential Stem Cells. J. Vis. Exp. (191), e64608, doi:10.3791/64608 (2023).

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