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Generatie van Natural Killer Cellen uit Human Expanded Potential Stamcellen
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Generation of Natural Killer Cells from Human Expanded Potential Stem Cells
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Generatie van Natural Killer Cellen uit Human Expanded Potential Stamcellen

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2,461 Views

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06:53 min

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January 13, 2023

DOI:

10.3791/64608-v

DOI:
10.3791/64608-v

•

06:53 min
January 13, 2023

•

11 Views
, , , ,
1Major in Molecular Biology and Biotechnology, School of Biological Sciences,The University of Hong Kong, 2School of Biomedical Sciences,The University of Hong Kong, 3Centre for Translational Stem Cell Biology

Summary

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Het huidige protocol laat zien hoe CD3−/CD45+CD56+ cellen met milde cytotoxiciteit kunnen worden onderscheiden van humane expanded potential stamcellen (hEPSC's) onder zowel 3D- als 2D-kweekomstandigheden. Dit maakt routinematige fenotypische validatie mogelijk zonder de vernietiging van de complexe micro-omgeving.

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Yu Ching, P., Wang, C., Hang, S., Liu, P., Sugimura, R. Generation of Natural Killer Cells from Human Expanded Potential Stem Cells. J. Vis. Exp. (191), e64608, doi:10.3791/64608 (2023).

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