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Generation of Natural Killer Cells from Human Expanded Potential Stem Cells
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Generation of Natural Killer Cells from Human Expanded Potential Stem Cells

Generation of Natural Killer Cells from Human Expanded Potential Stem Cells

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06:53 min

January 13, 2023

DOI:

06:53 min
January 13, 2023

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Summary

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The present protocol shows how to differentiate CD3−/CD45+CD56+ cells with mild cytotoxicity from human expanded potential stem cells (hEPSCs) under both 3D and 2D culture conditions. This allows for routine phenotypical validation without the destruction of the complex microenvironment.

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