Journal
/
Developmental Biology
/
Generation of Natural Killer Cells from Human Expanded Potential Stem Cells
JoVE Journal
Developmental Biology
This content is Free Access.
JoVE Journal Developmental Biology
Generation of Natural Killer Cells from Human Expanded Potential Stem Cells

Generation of Natural Killer Cells from Human Expanded Potential Stem Cells

English

Automatically Generated

2,455 Views

•

06:53 min

•

January 13, 2023

DOI:

10.3791/64608-v

DOI:
10.3791/64608-v

•

06:53 min
January 13, 2023

•

11 Views
, , , ,
1Major in Molecular Biology and Biotechnology, School of Biological Sciences,The University of Hong Kong, 2School of Biomedical Sciences,The University of Hong Kong, 3Centre for Translational Stem Cell Biology

Summary

Automatically generated

The present protocol shows how to differentiate CD3−/CD45+CD56+ cells with mild cytotoxicity from human expanded potential stem cells (hEPSCs) under both 3D and 2D culture conditions. This allows for routine phenotypical validation without the destruction of the complex microenvironment.

Explore More Videos

Natural Killer CellsHuman Expanded Potential Stem CellsCancer ImmunotherapyEmbryoid BodiesMesoderm PatterningHematoendothelial SpecificationDifferentiationCell CultureCell SuspensionCell CountingCell Harvesting

Read Article

Cite this Article

Yu Ching, P., Wang, C., Hang, S., Liu, P., Sugimura, R. Generation of Natural Killer Cells from Human Expanded Potential Stem Cells. J. Vis. Exp. (191), e64608, doi:10.3791/64608 (2023).

Download .ris file

Copy

Share Video

.

Copy