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Journal
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Biochemistry
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使用新型3D打印支架对完整的
离体
地球仪进行活细胞成像
JoVE Journal
Biochemistry
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JoVE Journal
Biochemistry
Live-Cell Imaging of Intact
Ex Vivo
Globes Using a Novel 3D Printed Holder
Please note that all translations are automatically generated.
Click here for the English version.
使用新型3D打印支架对完整的
离体
地球仪进行活细胞成像
DOI:
10.3791/64510-v
•
05:41 min
•
October 06, 2022
•
Kristen L. Segars*
1
,
Nicholas A. Azzari*
2
,
Stephanie Gomez
,
Celeste B. Rich
,
Vickery Trinkaus-Randall
3
1
Department of Pharmacology, School of Medicine
,
Boston University
,
2
Department of Biochemistry, School of Medicine
,
Boston University
,
3
Department of Ophthalmology, School of Medicine
,
Boston University
Chapters
00:04
Introduction
00:48
Sample Collection and Preparation of Sample Holders
02:03
Wounding of the Eyes
02:54
Sample Placement on the Holder and Sample Imaging
04:18
Results: High‐Resolution Live Cell Imaging of Enucleated Globes
05:14
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
这项工作描述了一种新的实验方案,该方案利用3D打印支架来实现去核球的高分辨率活细胞成像。通过该协议,可以实时观察离
体
球受伤角膜上皮中的细胞钙信号活性。
Tags
Keywords: 3D Printed Holder
Live-cell Imaging
Ex Vivo Globes
Corneal Cells
Epithelium
Nerves
Wound Healing
Calcium Indicator
Cell Membrane Stain
Scratch Wound
Puncture Wound
Corneal Basement Membrane
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