Journal
/
Biochemistry
/
Fluorescence Live-cell Imaging of the Complete Vegetative Cell Cycle of the Slow-growing Social Bacterium Myxococcus xanthus
JoVE Journal
Biochemistry
This content is Free Access.
JoVE Journal Biochemistry
Fluorescence Live-cell Imaging of the Complete Vegetative Cell Cycle of the Slow-growing Social Bacterium Myxococcus xanthus

Fluorescence Live-cell Imaging of the Complete Vegetative Cell Cycle of the Slow-growing Social Bacterium Myxococcus xanthus

DOI:
10.3791/57860-v

•

11:45 min

•

June 20, 2018

•

,
1Department of Ecophysiology,Max Planck Institute for Terrestrial Microbiology

Chapters

  • 00:04Title
  • 00:39Growth and Preparation of M. xanthus Microscopy Samples
  • 02:41Microscope Set-up and Time-lapse Acquisition
  • 07:37Generation of Time-lapse Movies and Image Alignment
  • 09:16Results: Fluorescence Time-lapse Imaging of Myxococcus xanthus
  • 11:01Conclusion

Summary

Automatic Translation

Automatic Translation

Bacterial cells are spatially highly organized. To follow this organization over time in slow growing Myxococcus xanthus cells, a set-up for fluorescence live-cell imaging with high spatiotemporal resolution over several generations was developed. Using this method, spatiotemporal dynamics of important proteins for chromosome segregation and cell division could be determined.

Tags

Fluorescence Live-cell ImagingMyxococcus XanthusBacterial Cell BiologyCell ReplicationCell GrowthCell DivisionSlow-growing Bacterium1% CTT MediumAgarose Microscopy SolutionTPM BufferFluorescent MicrospheresTime-lapse Microscopy

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Identification of Small Molecule-binding Proteins in a Native Cellular Environment by Live-cell Photoaffinity Labeling

Purification of Biotinylated Cell Surface Proteins from Rhipicephalus microplus Epithelial Gut Cells

Reconstitution of Cell-cycle Oscillations in Microemulsions of Cell-free Xenopus Egg Extracts

A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts

Studying RNA Interactors of Protein Kinase RNA-Activated during the Mammalian Cell Cycle

Imaging of Extracellular Vesicles by Atomic Force Microscopy

Visualizing Surface T-Cell Receptor Dynamics Four-Dimensionally Using Lattice Light-Sheet Microscopy

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

Cell Culture on Silicon Nitride Membranes and Cryopreparation for Synchrotron X-ray Fluorescence Nano-analysis

Conventional BODIPY Conjugates for Live-Cell Super-Resolution Microscopy and Single-Molecule Tracking

Read Article