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Live-Cell Imaging of Intact Ex Vivo Globes Using a Novel 3D Printed Holder
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Biochemistry
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JoVE Journal Biochemistry
Live-Cell Imaging of Intact Ex Vivo Globes Using a Novel 3D Printed Holder

Live-Cell Imaging of Intact Ex Vivo Globes Using a Novel 3D Printed Holder

English

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2,471 Views

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05:41 min

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October 06, 2022

DOI:

10.3791/64510-v

DOI:
10.3791/64510-v

•

05:41 min
October 06, 2022

•

1 Views
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1Department of Pharmacology, School of Medicine,Boston University, 2Department of Biochemistry, School of Medicine,Boston University, 3Department of Ophthalmology, School of Medicine,Boston University

Summary

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The present work describes a novel experimental protocol that utilizes a 3D printed holder to enable high-resolution live cell imaging of enucleated globes. Through this protocol, the cellular calcium signaling activity in wounded corneal epithelium from ex vivo globes can be observed in real time.

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Cite this Article

Segars, K. L., Azzari, N. A., Gomez, S., Rich, C. B., Trinkaus-Randall, V. Live-Cell Imaging of Intact Ex Vivo Globes Using a Novel 3D Printed Holder. J. Vis. Exp. (188), e64510, doi:10.3791/64510 (2022).

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