Journal
/
生物化学
/
Live-Cell Imaging of Intact Ex Vivo Globes Using a Novel 3D Printed Holder
JoVE 杂志
生物化学
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 生物化学
Live-Cell Imaging of Intact Ex Vivo Globes Using a Novel 3D Printed Holder

Live-Cell Imaging of Intact Ex Vivo Globes Using a Novel 3D Printed Holder

2,471 Views

•

05:41 min

•

October 06, 2022

DOI:

10.3791/64510-v

DOI:
10.3791/64510-v

•

05:41 min
October 06, 2022

•

1 Views
, , , ,
1Department of Pharmacology, School of Medicine,Boston University, 2Department of Biochemistry, School of Medicine,Boston University, 3Department of Ophthalmology, School of Medicine,Boston University

Summary

Automatically generated

The present work describes a novel experimental protocol that utilizes a 3D printed holder to enable high-resolution live cell imaging of enucleated globes. Through this protocol, the cellular calcium signaling activity in wounded corneal epithelium from ex vivo globes can be observed in real time.

Explore More Videos

3D Printed HolderLive-cell ImagingEx Vivo GlobesCorneal CellsEpitheliumNervesWound HealingCalcium IndicatorCell Membrane StainScratch WoundPuncture WoundCorneal Basement Membrane

Read Article

Cite this Article

Segars, K. L., Azzari, N. A., Gomez, S., Rich, C. B., Trinkaus-Randall, V. Live-Cell Imaging of Intact Ex Vivo Globes Using a Novel 3D Printed Holder. J. Vis. Exp. (188), e64510, doi:10.3791/64510 (2022).

Download .ris file

Copy

Share Video

.

Copy