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Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing
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Genetics
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JoVE Journal Genetics
Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing

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1,884 Views

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09:44 min

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December 23, 2022

DOI:

10.3791/64404-v

DOI:
10.3791/64404-v

•

09:44 min
December 23, 2022

•

2 Views
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1Unidad de Investigaciones en Salud Pública “Dra. Kaethe Willms”, Facultad de Ciencias Químico Biológicas,Universidad Autónoma de Sinaloa, 2Centro de Investigación en Alimentación y Desarrollo, A. C. (CIAD),Unidad Mazatlán en Acuicultura y Manejo Ambiental

Summary

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The feasibility of whole-genome sequencing (WGS) strategies using benchtop instruments has simplified the genome interrogation of every microbe of public health relevance in a lab setting. A methodological adaptation of the workflow for bacterial WGS is described and a bioinformatics pipeline for analysis is also presented.

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Whole-genome SequencingWGSAntimicrobial ResistancePathogenic Escherichia ColiOreochromis Spp. FarmsNext-generation SequencingDNA ExtractionTagmentationIndexingPCRMagnetic Bead Purification

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Cite this Article

Magaña-Lizárraga, J. A., Gómez-Gil, B., Enciso-Ibarra, J., Báez-Flores, M. E. Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing. J. Vis. Exp. (190), e64404, doi:10.3791/64404 (2022).

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