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Immunology and Infection
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Flowcytometribaseret kvantificering og analyse af myokardie-B-celler
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Immunology and Infection
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JoVE Journal Immunology and Infection
Flow Cytometry-Based Quantification and Analysis of Myocardial B-Cells

Flowcytometribaseret kvantificering og analyse af myokardie-B-celler

DOI:
10.3791/64344-v

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12:46 min

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August 17, 2022

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, ,
1Division of Cardiology, Department of Medicine,The Johns Hopkins University School of Medicine

Chapters

  • 00:04Introduction
  • 01:05Intravascular B-Cell Staining and Heart Collection
  • 02:58Heart Digestion and Cell Staining
  • 05:35Flow Cytometry
  • 11:31Results: Representative Plots Reporting the Average Number of B-Cells Per Milligram of Myocardial Tissue
  • 12:12Conclusion

Summary

Automatic Translation

Automatic Translation

Her rapporterer vi en protokol til kvantificering og differentiering af myokardie B-lymfocytter baseret på deres placering i det intravaskulære eller endotelrum ved hjælp af flowcytometri.

Tags

Flow CytometryMyocardial B-cellsPerfusionDigestionIsolationAntibody PanelImmune Cell TypesHeartLungLiverB220 AntibodyEuthanasiaHBSSMinced HeartEnzymesDNase ICollagenase IIHyaluronidase

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