Journal
/
Immunology and Infection
/
Flow Cytometry-Based Quantification and Analysis of Myocardial B-Cells
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Flow Cytometry-Based Quantification and Analysis of Myocardial B-Cells

Flow Cytometry-Based Quantification and Analysis of Myocardial B-Cells

English

Automatically Generated

2,198 Views

•

12:46 min

•

August 17, 2022

DOI:

10.3791/64344-v

DOI:
10.3791/64344-v

•

12:46 min
August 17, 2022

•

4 Views
, ,
1Division of Cardiology, Department of Medicine,The Johns Hopkins University School of Medicine

Summary

Automatically generated

Here we report a protocol for the quantification and differentiation of myocardial B-lymphocytes based on their location in the intravascular or endothelial space using flow cytometry.

Explore More Videos

Flow CytometryMyocardial B-cellsPerfusionDigestionIsolationAntibody PanelImmune Cell TypesHeartLungLiverB220 AntibodyEuthanasiaHBSSMinced HeartEnzymesDNase ICollagenase IIHyaluronidase

Read Article

Cite this Article

Bermea, K. C., Rousseau, S. T., Adamo, L. Flow Cytometry-Based Quantification and Analysis of Myocardial B-Cells. J. Vis. Exp. (186), e64344, doi:10.3791/64344 (2022).

Download .ris file

Copy

Share Video

.

Copy