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Assessing Retinal Microglial Phagocytic Function In Vivo Using a Flow Cytometry-based Assay
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Immunology and Infection
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JoVE Journal Immunology and Infection
Assessing Retinal Microglial Phagocytic Function In Vivo Using a Flow Cytometry-based Assay

Assessing Retinal Microglial Phagocytic Function In Vivo Using a Flow Cytometry-based Assay

English

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9,468 Views

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07:19 min

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October 18, 2016

DOI:

10.3791/54677-v

DOI:
10.3791/54677-v

•

07:19 min
October 18, 2016

•

2 Views
, , , , ,
1Department of Cell and Molecular Biology,The Scripps Research Institute, 2Department of Chemical Physiology,The Scripps Research Institute, 3The Lowy Medical Research Institute

Summary

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Microglial phagocytosis is critical for the maintenance of tissue homeostasis and inadequate phagocytic function has been implicated in pathology. However, assessing microglia function in vivo is technically challenging. We have developed a simple but robust technique for precisely monitoring and quantifying the phagocytic potential of microglia in a physiological setting.

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Cite this Article

Murinello, S., Moreno, S. K., Macauley, M. S., Sakimoto, S., Westenskow, P. D., Friedlander, M. Assessing Retinal Microglial Phagocytic Function In Vivo Using a Flow Cytometry-based Assay. J. Vis. Exp. (116), e54677, doi:10.3791/54677 (2016).

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