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Assessing Retinal Microglial Phagocytic Function In Vivo Using a Flow Cytometry-based Assay
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Immunology and Infection
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JoVE Journal Immunology and Infection
Assessing Retinal Microglial Phagocytic Function In Vivo Using a Flow Cytometry-based Assay

Assessing Retinal Microglial Phagocytic Function In Vivo Using a Flow Cytometry-based Assay

DOI:
10.3791/54677-v

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07:19 min

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October 18, 2016

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, , , , ,
1Department of Cell and Molecular Biology,The Scripps Research Institute, 2Department of Chemical Physiology,The Scripps Research Institute, 3The Lowy Medical Research Institute

Chapters

  • 00:05Title
  • 00:47Intravitreal Bead Injection
  • 02:48Retinal Tissue Harvest
  • 04:19Single Cell Suspension Staining for Flow Cytometric Analysis
  • 05:49Results: Representative Microglia Phagocytic Function after LPS Challenge in Pup and Adult Mice
  • 06:30Conclusion

Summary

Automatic Translation

Automatic Translation

Microglial phagocytosis is critical for the maintenance of tissue homeostasis and inadequate phagocytic function has been implicated in pathology. However, assessing microglia function in vivo is technically challenging. We have developed a simple but robust technique for precisely monitoring and quantifying the phagocytic potential of microglia in a physiological setting.

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Retinal MicrogliaPhagocytic FunctionFlow CytometryIntravitreal InjectionFluorescent ParticlesEye Dissection

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