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Journal
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Immunology and Infection
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基于流式细胞术的心肌B细胞定量和分析
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Flow Cytometry-Based Quantification and Analysis of Myocardial B-Cells
Please note that all translations are automatically generated.
Click here for the English version.
基于流式细胞术的心肌B细胞定量和分析
DOI:
10.3791/64344-v
•
12:46 min
•
August 17, 2022
•
Kevin C. Bermea
,
Sylvie T. Rousseau
,
Luigi Adamo
1
Division of Cardiology, Department of Medicine
,
The Johns Hopkins University School of Medicine
Chapters
00:04
Introduction
01:05
Intravascular B-Cell Staining and Heart Collection
02:58
Heart Digestion and Cell Staining
05:35
Flow Cytometry
11:31
Results: Representative Plots Reporting the Average Number of B-Cells Per Milligram of Myocardial Tissue
12:12
Conclusion
Summary
Automatic Translation
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在这里,我们报告了一种使用流式细胞术根据心肌B淋巴细胞在血管内或内皮空间中的位置对心肌B淋巴细胞进行定量和分化的协议。
Tags
Flow Cytometry
Myocardial B-cells
Perfusion
Digestion
Isolation
Antibody Panel
Immune Cell Types
Heart
Lung
Liver
B220 Antibody
Euthanasia
HBSS
Minced Heart
Enzymes
DNase I
Collagenase II
Hyaluronidase
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