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Isolamento e quantificação do vírus Epstein-Barr a partir da linhagem celular P3HR1
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Immunology and Infection
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JoVE Journal Immunology and Infection
Isolation and Quantification of Epstein-Barr Virus from the P3HR1 Cell Line

Isolamento e quantificação do vírus Epstein-Barr a partir da linhagem celular P3HR1

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3,255 Views

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09:14 min

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September 28, 2022

DOI:

10.3791/64279-v

DOI:
10.3791/64279-v

•

09:14 min
September 28, 2022

•

16 Views
, ,
1Department of Experimental Pathology, Immunology and Microbiology, Faculty of Medicine,American University of Beirut, 2Center for Infectious Diseases Research (CIDR), Faculty of Medicine,American University of Beirut

Summary

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Este protocolo permite o isolamento de partículas do vírus Epstein-Barr da linhagem celular humana P3HR1 ao induzir o ciclo lítico viral com 12-miristato de forbol 13-acetato. O DNA é subsequentemente extraído da preparação viral e submetido a PCR em tempo real para quantificar a concentração de partículas virais.

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Cite this Article

Bitar, E. R., Shams Eddin, M. S., Rahal, E. A. Isolation and Quantification of Epstein-Barr Virus from the P3HR1 Cell Line. J. Vis. Exp. (187), e64279, doi:10.3791/64279 (2022).

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