Journal
/
免疫与感染
/
Isolation and Quantification of Epstein-Barr Virus from the P3HR1 Cell Line
JoVE 杂志
免疫与感染
Author Produced
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 免疫与感染
Isolation and Quantification of Epstein-Barr Virus from the P3HR1 Cell Line

Isolation and Quantification of Epstein-Barr Virus from the P3HR1 Cell Line

DOI:
10.3791/64279-v

•

09:14 min

•

September 28, 2022

•

, ,
1Department of Experimental Pathology, Immunology and Microbiology, Faculty of Medicine,American University of Beirut, 2Center for Infectious Diseases Research (CIDR), Faculty of Medicine,American University of Beirut

Chapters

  • 00:05Introduction
  • 00:36Preparing the Plate for Culture
  • 01:49Induction and Isolation of Epstein-Barr Virus Particles
  • 02:45DNA Extraction from Viral Particles
  • 04:45Checking DNA Concentration and Purity
  • 05:34Quantification by Real-Time Polymerase Chain Reaction
  • 07:54Results: Cell Counting and Induction of P3HR-1 Cells with PMA and DMSO
  • 08:48Conclusion

Summary

自动翻译

自动翻译

This protocol permits the isolation of Epstein-Barr virus particles from the human P3HR1 cell line upon inducing the viral lytic cycle with phorbol 12-myristate 13-acetate. DNA is subsequently extracted from the viral preparation and subjected to real-time PCR to quantify the viral particle concentration.

Tags

Keywords: Epstein-Barr VirusP3HR1 Cell LineViral StockVirus IsolationVirus QuantificationPMADMSOCell CultureCentrifugationPhenol-chloroform ExtractionEthanol Precipitation

Article

Embed

添加到播放列表

Usage Statistics

Related Videos

check_url/cn/64279?article_type=v

Detection of Infectious Virus from Field-collected Mosquitoes by Vero Cell Culture Assay
check_url/cn/64279?article_type=v

Establishment of Epstein-Barr Virus Growth-transformed Lymphoblastoid Cell Lines
check_url/cn/64279?article_type=v

Expanding Cytotoxic T Lymphocytes from Umbilical Cord Blood that Target Cytomegalovirus, Epstein-Barr Virus, and Adenovirus
check_url/cn/64279?article_type=v

Isolation and Genome Analysis of Single Virions using ‘Single Virus Genomics’
check_url/cn/64279?article_type=v

Nasal Wipes for Influenza A Virus Detection and Isolation from Swine
check_url/cn/64279?article_type=v

Isolation and Flow Cytometric Analysis of Immune Cells from the Ischemic Mouse Brain
check_url/cn/64279?article_type=v

Neutrophil Isolation and Analysis to Determine their Role in Lymphoma Cell Sensitivity to Therapeutic Agents
check_url/cn/64279?article_type=v

Evaluation of the Efficacy And Toxicity of RNAs Targeting HIV-1 Production for Use in Gene or Drug Therapy
check_url/cn/64279?article_type=v

An Efficient and Simple Method to Establish NK and T Cell Lines from Patients with Chronic Active Epstein-Barr Virus Infection
check_url/cn/64279?article_type=v

Quantification of Efferocytosis by Single-cell Fluorescence Microscopy

Read Article