/
/
Isolation and Quantification of Epstein-Barr Virus from the P3HR1 Cell Line
Bu içeriği görüntülemek için JoVE aboneliği gereklidir. Oturum açın veya ücretsiz deneme sürümünü başlatın.
Isolation and Quantification of Epstein-Barr Virus from the P3HR1 Cell Line
3,255 Views
•
09:14 min
•
September 28, 2022
DOI:
Özet
Automatically generated
This protocol permits the isolation of Epstein-Barr virus particles from the human P3HR1 cell line upon inducing the viral lytic cycle with phorbol 12-myristate 13-acetate. DNA is subsequently extracted from the viral preparation and subjected to real-time PCR to quantify the viral particle concentration.
Read Article
Cite this Article
Bitar, E. R., Shams Eddin, M. S., Rahal, E. A. Isolation and Quantification of Epstein-Barr Virus from the P3HR1 Cell Line. J. Vis. Exp. (187), e64279, doi:10.3791/64279 (2022).
Copy