Journal
/
Immunology and Infection
/
Isolamento e quantificazione del virus Epstein-Barr dalla linea cellulare P3HR1
JoVE Journal
Immunology and Infection
Author Produced
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Isolation and Quantification of Epstein-Barr Virus from the P3HR1 Cell Line
Please note that all translations are automatically generated. Click here for the English version.

Isolamento e quantificazione del virus Epstein-Barr dalla linea cellulare P3HR1

italiano

Automatically Generated

3,255 Views

•

09:14 min

•

September 28, 2022

DOI:

10.3791/64279-v

DOI:
10.3791/64279-v

•

09:14 min
September 28, 2022

•

16 Views
, ,
1Department of Experimental Pathology, Immunology and Microbiology, Faculty of Medicine,American University of Beirut, 2Center for Infectious Diseases Research (CIDR), Faculty of Medicine,American University of Beirut

Summary

Automatically generated

Questo protocollo consente l'isolamento delle particelle virali di Epstein-Barr dalla linea cellulare umana P3HR1 inducendo il ciclo litico virale con forbolo 12-miristato 13-acetato. Il DNA viene successivamente estratto dalla preparazione virale e sottoposto a PCR in tempo reale per quantificare la concentrazione di particelle virali.

Explore More Videos

Epstein-Barr VirusP3HR1 Cell LineViral StockVirus IsolationVirus QuantificationPMADMSOCell CultureCentrifugationPhenol-chloroform ExtractionEthanol Precipitation

Read Article

Cite this Article

Bitar, E. R., Shams Eddin, M. S., Rahal, E. A. Isolation and Quantification of Epstein-Barr Virus from the P3HR1 Cell Line. J. Vis. Exp. (187), e64279, doi:10.3791/64279 (2022).

Download .ris file

Copy

Share Video

.

Copy