The present protocol describes the use of Isothermal Titration Calorimetry (ITC) to analyze the association and dissociation kinetics of the binding between a DNA aptamer and tetracycline, including sample preparation, running standards and samples, and interpreting the resulting data.
Thoa, T. T. T., Liao, A. M., Caltagirone, G. T. Determining the Thermodynamic and Kinetic Association of a DNA Aptamer and Tetracycline Using Isothermal Titration Calorimetry. J. Vis. Exp. (186), e64247, doi:10.3791/64247 (2022).