Journal
/
Biochemistry
/
İzotermal Titrasyon Kalorimetrisi Kullanılarak DNA Aptamer ve Tetrasiklinin Termodinamik ve Kinetik İlişkisinin Belirlenmesi
JoVE Journal
Biochemistry
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biochemistry
Determining the Thermodynamic and Kinetic Association of a DNA Aptamer and Tetracycline Using Isothermal Titration Calorimetry
Please note that all translations are automatically generated. Click here for the English version.

İzotermal Titrasyon Kalorimetrisi Kullanılarak DNA Aptamer ve Tetrasiklinin Termodinamik ve Kinetik İlişkisinin Belirlenmesi

2,544 Views

•

06:02 min

•

August 23, 2022

DOI:

10.3791/64247-v

DOI:
10.3791/64247-v

•

06:02 min
August 23, 2022

•

1 Views
, ,
1Aptagen LLC

Tags

DNA AptamerTetracyclineIsothermal Titration Calorimetry (ITC)Binding AffinityThermodynamic And Kinetic AssociationBuffer ExchangeAptamer FoldingDegassingITC Measurement

Read Article

Cite this Article

Thoa, T. T. T., Liao, A. M., Caltagirone, G. T. Determining the Thermodynamic and Kinetic Association of a DNA Aptamer and Tetracycline Using Isothermal Titration Calorimetry. J. Vis. Exp. (186), e64247, doi:10.3791/64247 (2022).

Download .ris file

Copy

Share Video

.

Copy