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Determining the Thermodynamic and Kinetic Association of a DNA Aptamer and Tetracycline Using Isothermal Titration Calorimetry
JoVE 杂志
生物化学
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JoVE 杂志 生物化学
Determining the Thermodynamic and Kinetic Association of a DNA Aptamer and Tetracycline Using Isothermal Titration Calorimetry

Determining the Thermodynamic and Kinetic Association of a DNA Aptamer and Tetracycline Using Isothermal Titration Calorimetry

DOI:
10.3791/64247-v

•

06:02 min

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August 23, 2022

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, ,
1Aptagen LLC

Chapters

  • 00:04Introduction
  • 00:40Preparation of Samples
  • 02:23Running the Test Kit and the Sample to Determine the Binding Between Aptamer and Tetracycline
  • 03:26Analyzing Data Using Software
  • 04:22Results: ITC Thermogram of a DNA Aptamer and Tetracycline
  • 05:23Conclusion

Summary

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The present protocol describes the use of Isothermal Titration Calorimetry (ITC) to analyze the association and dissociation kinetics of the binding between a DNA aptamer and tetracycline, including sample preparation, running standards and samples, and interpreting the resulting data.

Tags

DNA AptamerTetracyclineIsothermal Titration Calorimetry (ITC)Binding AffinityThermodynamic And Kinetic AssociationBuffer ExchangeAptamer FoldingDegassingITC Measurement

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