Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich. Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
Determining the Thermodynamic and Kinetic Association of a DNA Aptamer and Tetracycline Using Isothermal Titration Calorimetry
2,591 Views
•
06:02 min
•
August 23, 2022
DOI:
Summary
Automatically generated
The present protocol describes the use of Isothermal Titration Calorimetry (ITC) to analyze the association and dissociation kinetics of the binding between a DNA aptamer and tetracycline, including sample preparation, running standards and samples, and interpreting the resulting data.
Read Article
Cite this Article
Thoa, T. T. T., Liao, A. M., Caltagirone, G. T. Determining the Thermodynamic and Kinetic Association of a DNA Aptamer and Tetracycline Using Isothermal Titration Calorimetry. J. Vis. Exp. (186), e64247, doi:10.3791/64247 (2022).
Copy