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Depolarizzazione selettiva assistita da microfluidica dei mitocondri assonali
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Neuroscience
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JoVE Journal Neuroscience
Microfluidics-Assisted Selective Depolarization of Axonal Mitochondria
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Depolarizzazione selettiva assistita da microfluidica dei mitocondri assonali

italiano

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1,587 Views

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06:55 min

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August 04, 2022

DOI:

10.3791/64196-v

DOI:
10.3791/64196-v

•

06:55 min
August 04, 2022

•

3 Views
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1TUM Medical Graduate Center,Technical University of Munich, 2Max Planck Institute of Neurobiology, 3TUM School of Medicine, Institute of Neuronal Cell Biology,Technical University of Munich, 4Munich Cluster for Systems Neurology

Summary

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Il presente protocollo descrive la semina e la colorazione dei mitocondri neuronali in camere microfluidiche. Il gradiente di pressione fluidica in queste camere consente il trattamento selettivo dei mitocondri negli assoni per analizzare le loro proprietà in risposta alle sfide farmacologiche senza influenzare il compartimento corporeo cellulare.

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Cite this Article

Wanderoy, S., Rühmkorf, A., Harbauer, A. B. Microfluidics-Assisted Selective Depolarization of Axonal Mitochondria. J. Vis. Exp. (186), e64196, doi:10.3791/64196 (2022).

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