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Microfluidics-Assisted Selective Depolarization of Axonal Mitochondria
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JoVE Journal Nörobilim
Microfluidics-Assisted Selective Depolarization of Axonal Mitochondria

Microfluidics-Assisted Selective Depolarization of Axonal Mitochondria

1,587 Views

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06:55 min

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August 04, 2022

DOI:

10.3791/64196-v

DOI:
10.3791/64196-v

•

06:55 min
August 04, 2022

•

3 Views
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1TUM Medical Graduate Center,Technical University of Munich, 2Max Planck Institute of Neurobiology, 3TUM School of Medicine, Institute of Neuronal Cell Biology,Technical University of Munich, 4Munich Cluster for Systems Neurology

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The present protocol describes the seeding and staining of neuronal mitochondria in microfluidic chambers. The fluidic pressure gradient in these chambers allows for the selective treatment of mitochondria in axons to analyze their properties in response to pharmacological challenges without affecting the cell body compartment.

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MikroakışkanlarAxonal MitochondriaNeurodegenerative DiseasesFluidic Pressure GradientMembrane PotentialHippocampal NeuronsCell CultureB-27 Neurobasal MediaMicrogrooves

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Wanderoy, S., Rühmkorf, A., Harbauer, A. B. Microfluidics-Assisted Selective Depolarization of Axonal Mitochondria. J. Vis. Exp. (186), e64196, doi:10.3791/64196 (2022).

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