Journal
/
/
Microfluidics-Assisted Selective Depolarization of Axonal Mitochondria
JoVE Journal
Neuroscience
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Neuroscience
Microfluidics-Assisted Selective Depolarization of Axonal Mitochondria

Microfluidics-Assisted Selective Depolarization of Axonal Mitochondria

1,590 Views

06:55 min

August 04, 2022

DOI:

06:55 min
August 04, 2022

3 Views
, ,

Summary

Automatically generated

The present protocol describes the seeding and staining of neuronal mitochondria in microfluidic chambers. The fluidic pressure gradient in these chambers allows for the selective treatment of mitochondria in axons to analyze their properties in response to pharmacological challenges without affecting the cell body compartment.

Read Article