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Microfluidics-Assisted Selective Depolarization of Axonal Mitochondria
JoVE Journal
神経科学
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JoVE Journal 神経科学
Microfluidics-Assisted Selective Depolarization of Axonal Mitochondria

Microfluidics-Assisted Selective Depolarization of Axonal Mitochondria

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06:55 min

August 04, 2022

DOI:

06:55 min
August 04, 2022

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概要

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The present protocol describes the seeding and staining of neuronal mitochondria in microfluidic chambers. The fluidic pressure gradient in these chambers allows for the selective treatment of mitochondria in axons to analyze their properties in response to pharmacological challenges without affecting the cell body compartment.

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