ジャーナル
/
神経科学
/
Microfluidics-Assisted Selective Depolarization of Axonal Mitochondria
JoVE Journal
神経科学
このコンテンツを視聴するには、JoVE 購読が必要です。  サインイン又は無料トライアルを申し込む。
JoVE Journal 神経科学
Microfluidics-Assisted Selective Depolarization of Axonal Mitochondria

Microfluidics-Assisted Selective Depolarization of Axonal Mitochondria

1,584 Views

•

06:55 min

•

August 04, 2022

DOI:

10.3791/64196-v

DOI:
10.3791/64196-v

•

06:55 min
August 04, 2022

•

3 Views
, ,
1TUM Medical Graduate Center,Technical University of Munich, 2Max Planck Institute of Neurobiology, 3TUM School of Medicine, Institute of Neuronal Cell Biology,Technical University of Munich, 4Munich Cluster for Systems Neurology

概要

Automatically generated

The present protocol describes the seeding and staining of neuronal mitochondria in microfluidic chambers. The fluidic pressure gradient in these chambers allows for the selective treatment of mitochondria in axons to analyze their properties in response to pharmacological challenges without affecting the cell body compartment.

Explore More Videos

微小流体力学Axonal MitochondriaNeurodegenerative DiseasesFluidic Pressure GradientMembrane PotentialHippocampal NeuronsCell CultureB-27 Neurobasal MediaMicrogrooves

Read Article

Cite this Article

Wanderoy, S., Rühmkorf, A., Harbauer, A. B. Microfluidics-Assisted Selective Depolarization of Axonal Mitochondria. J. Vis. Exp. (186), e64196, doi:10.3791/64196 (2022).

Download .ris file

Copy

Share Video

.

Copy