Journal
/
Developmental Biology
/
快速有效地监测完整斑马鱼胚胎中正常和异常胞嘧啶甲基化的时空监测
JoVE Journal
Developmental Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Developmental Biology
Rapid and Efficient Spatiotemporal Monitoring of Normal and Aberrant Cytosine Methylation within Intact Zebrafish Embryos
Please note that all translations are automatically generated. Click here for the English version.

快速有效地监测完整斑马鱼胚胎中正常和异常胞嘧啶甲基化的时空监测

DOI:
10.3791/64190-v

•

07:16 min

•

August 18, 2022

•

,
1Department of Environmental Sciences,University of California, Riverside

Chapters

  • 00:05Introduction
  • 01:02Zebrafish Embryo Collection and Chemical Exposure
  • 02:25Dechorionation of Embryos
  • 03:22Immunohistochemistry Using 5-mC-Specific Antibody
  • 04:46Automated Imaging of Embryos Within 96-Well Plates
  • 06:02Results: Optimization of the Custom Module for Detection of 5-mC
  • 06:42Conclusion

Summary

Automatic Translation

Automatic Translation

本文描述了一种快速有效地对完整斑马鱼胚胎内正常和异常胞嘧啶甲基化进行时空监测的方案。

Tags

Zebrafish Embryos5-MethylcytosineEpigenetic BiomarkerSpatiotemporal MonitoringCost-efficientRapidAutomated AcquisitionLow Sensitivity ScreeningChemical ExposureEnvironmental StimuliNCG MethodsParaformaldehyde FixationDechorionation

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

分离和斑马鱼胚胎单细胞的表征

从斑马鱼胚胎内源性受体亲和标记检测

生物传感运动神经元膜电位在活斑马鱼胚胎

诱导生活青蛙和斑马鱼胚胎缺氧

活完好斑马鱼的单细胞 Photoconversion

斑马鱼早期胚胎和肿瘤细胞电活动的可视化研究

使用mRNA电穿孔快速有效地表达Avian胚胎中的多种蛋白质

斑马鱼胚胎和幼虫的整个山免疫性化学

使用头太空气色谱对斑马鱼胚胎中的乙醇水平进行定量

斑马鱼卵巢卵巢中卵泡细胞和卵母细胞的分离

Read Article