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Rapid and Efficient Spatiotemporal Monitoring of Normal and Aberrant Cytosine Methylation within Intact Zebrafish Embryos
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Entwicklungsbiologie
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JoVE Journal Entwicklungsbiologie
Rapid and Efficient Spatiotemporal Monitoring of Normal and Aberrant Cytosine Methylation within Intact Zebrafish Embryos

Rapid and Efficient Spatiotemporal Monitoring of Normal and Aberrant Cytosine Methylation within Intact Zebrafish Embryos

DOI:
10.3791/64190-v

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07:16 min

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August 18, 2022

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,
1Department of Environmental Sciences,University of California, Riverside

Kapitel

  • 00:05Introduction
  • 01:02Zebrafish Embryo Collection and Chemical Exposure
  • 02:25Dechorionation of Embryos
  • 03:22Immunohistochemistry Using 5-mC-Specific Antibody
  • 04:46Automated Imaging of Embryos Within 96-Well Plates
  • 06:02Results: Optimization of the Custom Module for Detection of 5-mC
  • 06:42Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

This paper describes a protocol for the rapid and efficient spatiotemporal monitoring of normal and aberrant cytosine methylation within intact zebrafish embryos.

Tags

Zebrafish Embryos5-MethylcytosineEpigenetic BiomarkerSpatiotemporal MonitoringCost-efficientRapidAutomated AcquisitionLow Sensitivity ScreeningChemical ExposureEnvironmental StimuliNCG MethodsParaformaldehyde FixationDechorionation

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