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Rapid and Efficient Spatiotemporal Monitoring of Normal and Aberrant Cytosine Methylation within Intact Zebrafish Embryos
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发育生物学
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JoVE 杂志 发育生物学
Rapid and Efficient Spatiotemporal Monitoring of Normal and Aberrant Cytosine Methylation within Intact Zebrafish Embryos

Rapid and Efficient Spatiotemporal Monitoring of Normal and Aberrant Cytosine Methylation within Intact Zebrafish Embryos

DOI:
10.3791/64190-v

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07:16 min

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August 18, 2022

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,
1Department of Environmental Sciences,University of California, Riverside

Chapters

  • 00:05Introduction
  • 01:02Zebrafish Embryo Collection and Chemical Exposure
  • 02:25Dechorionation of Embryos
  • 03:22Immunohistochemistry Using 5-mC-Specific Antibody
  • 04:46Automated Imaging of Embryos Within 96-Well Plates
  • 06:02Results: Optimization of the Custom Module for Detection of 5-mC
  • 06:42Conclusion

Summary

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This paper describes a protocol for the rapid and efficient spatiotemporal monitoring of normal and aberrant cytosine methylation within intact zebrafish embryos.

Tags

Zebrafish Embryos5-MethylcytosineEpigenetic BiomarkerSpatiotemporal MonitoringCost-efficientRapidAutomated AcquisitionLow Sensitivity ScreeningChemical ExposureEnvironmental StimuliNCG MethodsParaformaldehyde FixationDechorionation

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