Development of Knock-Out Muscle Cell Lines using Lentivirus-Mediated CRISPR/Cas9 Gene Editing

Mathilde Beaufils; Amandine Tourel; Anne Petiot; Nicole B. Halmai; Dave J. Segal; John Rendu; Isabelle Marty

doi:10.3791/64114

Journal

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Genetics

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Entwicklung von Knock-out-Muskelzelllinien mittels Lentivirus-vermittelter CRISPR/Cas9-Gen-Editing

JoVE Journal
Genetics

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JoVE Journal Genetics

Development of Knock-Out Muscle Cell Lines using Lentivirus-Mediated CRISPR/Cas9 Gene Editing

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Entwicklung von Knock-out-Muskelzelllinien mittels Lentivirus-vermittelter CRISPR/Cas9-Gen-Editing

3,733 Views

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10:12 min

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June 16, 2022

DOI:

10.3791/64114-v

DOI:

10.3791/64114-v

•

10:12 min

June 16, 2022

•

2 Views

Mathilde Beaufils¹, Amandine Tourel¹, Anne Petiot¹, Nicole B. Halmai², Dave J. Segal², John Rendu¹, Isabelle Marty¹

¹University Grenoble Alpes, INSERM, U1216, CHU Grenoble Alpes, Grenoble Institut Neurosciences, ²Genome Center,University of California, Davis

Summary

Das Protokoll beschreibt, wie Knock-out-Myoblasten mit CRISPR/Cas9 erzeugt werden können, beginnend mit dem Design von Guide-RNAs bis hin zur zellulären Klonierung und Charakterisierung der Knock-out-Klone.

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Entwicklung von Knock-out-Muskelzelllinien mittels Lentivirus-vermittelter CRISPR/Cas9-Gen-Editing

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Summary

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