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实时和重复测量电活动改变的个体活斑马鱼的骨骼肌生长
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Developmental Biology
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JoVE Journal Developmental Biology
Real Time and Repeated Measurement of Skeletal Muscle Growth in Individual Live Zebrafish Subjected to Altered Electrical Activity
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实时和重复测量电活动改变的个体活斑马鱼的骨骼肌生长

DOI:
10.3791/64063-v

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11:41 min

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June 16, 2022

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, , ,
1Randall Centre for Cell and Molecular Biophysics, School of Basic and Medical Biosciences,King’s College London

Chapters

  • 00:05Introduction
  • 00:53Mounting Fish for Confocal Scanning
  • 03:05Confocal Scanning
  • 05:41Optional Method: Remove and Re-Introduce Muscle Electrical Activity
  • 09:01Results: Accurate and Efficient Estimation of the Muscle Volume of Live Zebrafish Larvae at Selected Stages of Activity
  • 11:01Conclusion

Summary

Automatic Translation

Automatic Translation

光学透明度是斑马鱼细胞生物学和生理学工作的主要优势。描述了用于测量个体动物细胞生长的稳健方法，该方法允许对骨骼肌和邻近组织的生长如何与全身生长相结合的新见解。

Tags

Skeletal Muscle GrowthLive ZebrafishAltered Electrical ActivityIn Vivo MeasurementReal-time MonitoringConfocal MicroscopyLow-melting AgaroseDevelopmental BiologyMuscle Wasting Disorders

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