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Real Time and Repeated Measurement of Skeletal Muscle Growth in Individual Live Zebrafish Subjected to Altered Electrical Activity
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Real Time and Repeated Measurement of Skeletal Muscle Growth in Individual Live Zebrafish Subjected to Altered Electrical Activity
Kapitel
- 00:05Introduction
- 00:53Mounting Fish for Confocal Scanning
- 03:05Confocal Scanning
- 05:41Optional Method: Remove and Re-Introduce Muscle Electrical Activity
- 09:01Results: Accurate and Efficient Estimation of the Muscle Volume of Live Zebrafish Larvae at Selected Stages of Activity
- 11:01Conclusion
Optical clarity is a major advantage for cell biological and physiological work in zebrafish. Robust methods for measurement of cell growth in individual animals are described that permit novel insights into how growth of skeletal muscle and neighboring tissues are integrated with whole body growth.
