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Un método biológico semiautomatizado y reproducible para cuantificar la deposición de calcio in vitro
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
A Semi-Automated and Reproducible Biological-Based Method to Quantify Calcium Deposition In Vitro
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Un método biológico semiautomatizado y reproducible para cuantificar la deposición de calcio in vitro

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1,826 Views

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11:30 min

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June 02, 2022

DOI:

10.3791/64029-v

DOI:
10.3791/64029-v

•

11:30 min
June 02, 2022

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2 Views
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1Department of Biochemistry, Cardiovascular Research Institute Maastricht (CARIM),Maastricht University, 2Helmholtz Institute for Biomedical Engineering, Biointerface Group,RWTH Aachen University, 3Institute of Experimental Medicine and Systems Biology,RWTH Aachen University

Summary

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Las enfermedades cardiovasculares son la principal causa de muerte en todo el mundo. La calcificación vascular contribuye sustancialmente a la carga de morbilidad y mortalidad cardiovascular. Este protocolo describe un método simple para cuantificar la precipitación de calcio mediada por células del músculo liso vascular in vitro mediante imágenes fluorescentes.

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Cite this Article

Jaminon, A. M. G., Rapp, N., Akbulut, A. C., Dzhanaev, R., Reutelingsperger, C. P., Jahnen-Dechent, W., Schurgers, L. J. A Semi-Automated and Reproducible Biological-Based Method to Quantify Calcium Deposition In Vitro. J. Vis. Exp. (184), e64029, doi:10.3791/64029 (2022).

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