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A Semi-Automated and Reproducible Biological-Based Method to Quantify Calcium Deposition In Vitro
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A Semi-Automated and Reproducible Biological-Based Method to Quantify Calcium Deposition In Vitro
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June 02, 2022
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Cardiovascular disease is the leading cause of death worldwide. Vascular calcification contributes substantially to the burden of cardiovascular morbidity and mortality. This protocol describes a simple method to quantify vascular smooth muscle cell-mediated calcium precipitation in vitro by fluorescent imaging.
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Jaminon, A. M. G., Rapp, N., Akbulut, A. C., Dzhanaev, R., Reutelingsperger, C. P., Jahnen-Dechent, W., Schurgers, L. J. A Semi-Automated and Reproducible Biological-Based Method to Quantify Calcium Deposition In Vitro. J. Vis. Exp. (184), e64029, doi:10.3791/64029 (2022).
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