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A Semi-Automated and Reproducible Biological-Based Method to Quantify Calcium Deposition In Vitro
JoVE Journal
Biochimica
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JoVE Journal Biochimica
A Semi-Automated and Reproducible Biological-Based Method to Quantify Calcium Deposition In Vitro
DOI:

11:30 min

June 02, 2022

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Capitoli

  • 00:05Introduction
  • 00:36Cell Seeding, Maintenance, and Calcification Induction
  • 02:13Calcification Detection via Imaging
  • 07:51Data Analysis
  • 10:13Results: A Semi-Automated Method for In Vitro Calcification Determination
  • 11:05Conclusion

Summary

Traduzione automatica

Cardiovascular disease is the leading cause of death worldwide. Vascular calcification contributes substantially to the burden of cardiovascular morbidity and mortality. This protocol describes a simple method to quantify vascular smooth muscle cell-mediated calcium precipitation in vitro by fluorescent imaging.

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