Journal
/
生物化学
/
A Semi-Automated and Reproducible Biological-Based Method to Quantify Calcium Deposition In Vitro
JoVE 杂志
生物化学
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 生物化学
A Semi-Automated and Reproducible Biological-Based Method to Quantify Calcium Deposition In Vitro

A Semi-Automated and Reproducible Biological-Based Method to Quantify Calcium Deposition In Vitro

1,826 Views

•

11:30 min

•

June 02, 2022

DOI:

10.3791/64029-v

DOI:
10.3791/64029-v

•

11:30 min
June 02, 2022

•

2 Views
, , , , , ,
1Department of Biochemistry, Cardiovascular Research Institute Maastricht (CARIM),Maastricht University, 2Helmholtz Institute for Biomedical Engineering, Biointerface Group,RWTH Aachen University, 3Institute of Experimental Medicine and Systems Biology,RWTH Aachen University

Summary

Automatically generated

Cardiovascular disease is the leading cause of death worldwide. Vascular calcification contributes substantially to the burden of cardiovascular morbidity and mortality. This protocol describes a simple method to quantify vascular smooth muscle cell-mediated calcium precipitation in vitro by fluorescent imaging.

Explore More Videos

CalcificationVascular Smooth Muscle CellsFluorescent-based DetectionHVSMCSCalcification MediumDAPIRFPBright FieldImagingData Reduction

Read Article

Cite this Article

Jaminon, A. M. G., Rapp, N., Akbulut, A. C., Dzhanaev, R., Reutelingsperger, C. P., Jahnen-Dechent, W., Schurgers, L. J. A Semi-Automated and Reproducible Biological-Based Method to Quantify Calcium Deposition In Vitro. J. Vis. Exp. (184), e64029, doi:10.3791/64029 (2022).

Download .ris file

Copy

Share Video

.

Copy