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RNA Fluorescence in situ Hybridization (FISH) to Visualize Microbial Colonization and Infection in Caenorhabditis elegans Intestines
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
RNA Fluorescence in situ Hybridization (FISH) to Visualize Microbial Colonization and Infection in Caenorhabditis elegans Intestines

RNA Fluorescence in situ Hybridization (FISH) to Visualize Microbial Colonization and Infection in Caenorhabditis elegans Intestines

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3,118 Views

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08:58 min

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July 27, 2022

DOI:

10.3791/63980-v

DOI:
10.3791/63980-v

•

08:58 min
July 27, 2022

•

18 Views
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1Department of Biology,San Diego State University, 2School of Biological Sciences,University of California, San Diego

Summary

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Intestinal microbes, including extracellular bacteria and intracellular pathogens like the Orsay virus and microsporidia (fungi), are often associated with wild Caenorhabditis nematodes. This article presents a protocol for detecting and quantifying microbes that colonize and/or infect C. elegans nematodes, and for measuring pathogen load after controlled infections in the lab.

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RNA FISHMicrobial ColonizationCaenorhabditis ElegansIntestinesMicrobiome BacteriaIntracellular PathogensVisualizationIdentificationQuantificationFixationHybridization BufferFISH Probe

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Rivera, D. E., Lažetić, V., Troemel, E. R., Luallen, R. J. RNA Fluorescence in situ Hybridization (FISH) to Visualize Microbial Colonization and Infection in Caenorhabditis elegans Intestines. J. Vis. Exp. (185), e63980, doi:10.3791/63980 (2022).

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