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RNA Fluorescence in situ Hybridization (FISH) to Visualize Microbial Colonization and Infection in Caenorhabditis elegans Intestines
JoVE Journal
免疫学と感染
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JoVE Journal 免疫学と感染
RNA Fluorescence in situ Hybridization (FISH) to Visualize Microbial Colonization and Infection in Caenorhabditis elegans Intestines

RNA Fluorescence in situ Hybridization (FISH) to Visualize Microbial Colonization and Infection in Caenorhabditis elegans Intestines

DOI:
10.3791/63980-v

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08:58 min

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July 27, 2022

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, , ,
1生物学科,San Diego State University, 2School of Biological Sciences,University of California, San Diego

章

  • 00:05Introduction
  • 00:41FISH Staining with PFA Fixation
  • 06:15Results: FISH Staining of Wild C. tropicalis JU1848 Strain and C. elegans
  • 08:14Conclusion

概要

自動翻訳

自動翻訳

Intestinal microbes, including extracellular bacteria and intracellular pathogens like the Orsay virus and microsporidia (fungi), are often associated with wild Caenorhabditis nematodes. This article presents a protocol for detecting and quantifying microbes that colonize and/or infect C. elegans nematodes, and for measuring pathogen load after controlled infections in the lab.

タグ

RNA FISHMicrobial ColonizationCaenorhabditis ElegansIntestinesMicrobiome BacteriaIntracellular PathogensVisualizationIdentificationQuantificationFixationHybridization BufferFISH Probe

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