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RNA Fluorescence in situ Hybridization (FISH) to Visualize Microbial Colonization and Infection in Caenorhabditis elegans Intestines
JoVE 杂志
免疫与感染
This content is Free Access.
JoVE 杂志 免疫与感染
RNA Fluorescence in situ Hybridization (FISH) to Visualize Microbial Colonization and Infection in Caenorhabditis elegans Intestines

RNA Fluorescence in situ Hybridization (FISH) to Visualize Microbial Colonization and Infection in Caenorhabditis elegans Intestines

DOI:
10.3791/63980-v

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08:58 min

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July 27, 2022

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, , ,
1Department of Biology,San Diego State University, 2School of Biological Sciences,University of California, San Diego

Chapters

  • 00:05Introduction
  • 00:41FISH Staining with PFA Fixation
  • 06:15Results: FISH Staining of Wild C. tropicalis JU1848 Strain and C. elegans
  • 08:14Conclusion

Summary

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Intestinal microbes, including extracellular bacteria and intracellular pathogens like the Orsay virus and microsporidia (fungi), are often associated with wild Caenorhabditis nematodes. This article presents a protocol for detecting and quantifying microbes that colonize and/or infect C. elegans nematodes, and for measuring pathogen load after controlled infections in the lab.

Tags

RNA FISHMicrobial ColonizationCaenorhabditis ElegansIntestinesMicrobiome BacteriaIntracellular PathogensVisualizationIdentificationQuantificationFixationHybridization BufferFISH Probe

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