Journal
/
Immunologie und Infektion
/
RNA Fluorescence in situ Hybridization (FISH) to Visualize Microbial Colonization and Infection in Caenorhabditis elegans Intestines
JoVE Journal
Immunologie und Infektion
This content is Free Access.
JoVE Journal Immunologie und Infektion
RNA Fluorescence in situ Hybridization (FISH) to Visualize Microbial Colonization and Infection in Caenorhabditis elegans Intestines

RNA Fluorescence in situ Hybridization (FISH) to Visualize Microbial Colonization and Infection in Caenorhabditis elegans Intestines

DOI:
10.3791/63980-v

•

08:58 min

•

July 27, 2022

•

, , ,
1Department of Biology,San Diego State University, 2School of Biological Sciences,University of California, San Diego

Kapitel

  • 00:05Introduction
  • 00:41FISH Staining with PFA Fixation
  • 06:15Results: FISH Staining of Wild C. tropicalis JU1848 Strain and C. elegans
  • 08:14Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

Intestinal microbes, including extracellular bacteria and intracellular pathogens like the Orsay virus and microsporidia (fungi), are often associated with wild Caenorhabditis nematodes. This article presents a protocol for detecting and quantifying microbes that colonize and/or infect C. elegans nematodes, and for measuring pathogen load after controlled infections in the lab.

Tags

RNA FISHMicrobial ColonizationCaenorhabditis ElegansIntestinesMicrobiome BacteriaIntracellular PathogensVisualizationIdentificationQuantificationFixationHybridization BufferFISH Probe

Article

Embed

ZUR WIEDERGABELISTE HINZUFÜGEN

Usage Statistics

Verwandte Videos

Combination of Adhesive-tape-based Sampling and Fluorescence in situ Hybridization for Rapid Detection of Salmonella on Fresh Produce

Locked Nucleic Acid Flow Cytometry-fluorescence in situ Hybridization (LNA flow-FISH): a Method for Bacterial Small RNA Detection

Use of Artificial Sputum Medium to Test Antibiotic Efficacy Against Pseudomonas aeruginosa in Conditions More Relevant to the Cystic Fibrosis Lung

Fluorescent in situ Hybridization on Mitotic Chromosomes of Mosquitoes

Fluorescence in situ Hybridizations (FISH) for the Localization of Viruses and Endosymbiotic Bacteria in Plant and Insect Tissues

Using Fluorescent Proteins to Visualize and Quantitate Chlamydia Vacuole Growth Dynamics in Living Cells

Complementary Use of Microscopic Techniques and Fluorescence Reading in Studying Cryptococcus-Amoeba Interactions

Evaluating Virulence and Pathogenesis of Aeromonas Infection in a Caenorhabditis elegans Model

Quantitative Fluorescence In Situ Hybridization (FISH) and Immunofluorescence (IF) of Specific Gene Products in KSHV-Infected Cells

Visualization of SARS-CoV-2 using Immuno RNA-Fluorescence In Situ Hybridization

Read Article