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使用原子力显微镜结合偏振显微镜测量肝硬度
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Measurement of Liver Stiffness Using Atomic Force Microscopy Coupled with Polarization Microscopy
Please note that all translations are automatically generated.
Click here for the English version.
使用原子力显微镜结合偏振显微镜测量肝硬度
DOI:
10.3791/63974-v
•
10:10 min
•
July 20, 2022
•
Srikant Ojha
,
Jan Pribyl
,
Simon Klimovic
3
,
Daniel Hadraba
,
Marketa Jirouskova
,
Martin Gregor
1
Laboratory of Integrative Biology
,
Institute of Molecular Genetics of the Czech Academy of Sciences
,
2
CEITEC MU
,
Masaryk University
,
3
Department of Biochemistry, Faculty of Science
,
Masaryk University
,
4
Laboratory of Biomathematics
,
Czech-BioImaging IPHYS, Institute of Physiology of the Czech Academy of Sciences
Chapters
00:04
Introduction
00:52
Sample Preparation II
01:47
Measurement
05:32
Data Analysis
07:52
Results: Measurement of the Elastic Moduli of Collagen‐Rich Areas
09:19
Conclusion
Summary
Automatic Translation
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Automatic Translation
我们提出了一种使用原子力显微镜测量正常和患病肝脏中富含胶原蛋白的区域的弹性模量的方案。同时使用偏振显微镜为定位肝脏切片中富含胶原蛋白的区域提供了高空间精度。
Tags
Liver Stiffness
Atomic Force Microscopy
Polarization Microscopy
Collagen
Fibrogenesis
Liver Fibrosis
Paraformaldehyde
PBS
AFM
Cantilever
Optical Calibration
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