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原子間力顕微鏡と偏光顕微鏡を組み合わせた肝臓のこわばり測定
JoVE Journal
Biology
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JoVE Journal Biology
Measurement of Liver Stiffness Using Atomic Force Microscopy Coupled with Polarization Microscopy
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原子間力顕微鏡と偏光顕微鏡を組み合わせた肝臓のこわばり測定

日本語

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3,176 Views

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10:10 min

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July 20, 2022

DOI:

10.3791/63974-v

DOI:
10.3791/63974-v

•

10:10 min
July 20, 2022

•

13 Views
, , , , ,
1Laboratory of Integrative Biology,Institute of Molecular Genetics of the Czech Academy of Sciences, 2CEITEC MU,Masaryk University, 3Department of Biochemistry, Faculty of Science,Masaryk University, 4Laboratory of Biomathematics,Czech-BioImaging IPHYS, Institute of Physiology of the Czech Academy of Sciences

Summary

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原子間力顕微鏡を使用して、正常および病気の肝臓のコラーゲンが豊富な領域の弾性率を測定するためのプロトコルを提示します。偏光顕微鏡を同時に使用することで、肝臓切片のコラーゲンが豊富な領域を特定するための高い空間精度が得られます。

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Cite this Article

Ojha, S., Pribyl, J., Klimovic, S., Hadraba, D., Jirouskova, M., Gregor, M. Measurement of Liver Stiffness Using Atomic Force Microscopy Coupled with Polarization Microscopy. J. Vis. Exp. (185), e63974, doi:10.3791/63974 (2022).

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