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Measurement of Liver Stiffness Using Atomic Force Microscopy Coupled with Polarization Microscopy
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July 20, 2022
DOI:
1Laboratory of Integrative Biology,Institute of Molecular Genetics of the Czech Academy of Sciences, 2CEITEC MU,Masaryk University, 3Department of Biochemistry, Faculty of Science,Masaryk University, 4Laboratory of Biomathematics,Czech-BioImaging IPHYS, Institute of Physiology of the Czech Academy of Sciences
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We present a protocol to measure the elastic moduli of collagen-rich areas in normal and diseased liver using atomic force microscopy. The simultaneous use of polarization microscopy provides high spatial precision for localizing collagen-rich areas in the liver sections.
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Ojha, S., Pribyl, J., Klimovic, S., Hadraba, D., Jirouskova, M., Gregor, M. Measurement of Liver Stiffness Using Atomic Force Microscopy Coupled with Polarization Microscopy. J. Vis. Exp. (185), e63974, doi:10.3791/63974 (2022).
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