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生物学
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Measurement of Liver Stiffness Using Atomic Force Microscopy Coupled with Polarization Microscopy
JoVE 杂志
生物学
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JoVE 杂志 生物学
Measurement of Liver Stiffness Using Atomic Force Microscopy Coupled with Polarization Microscopy

Measurement of Liver Stiffness Using Atomic Force Microscopy Coupled with Polarization Microscopy

DOI:
10.3791/63974-v

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10:10 min

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July 20, 2022

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, , , , ,
1Laboratory of Integrative Biology,Institute of Molecular Genetics of the Czech Academy of Sciences, 2CEITEC MU,Masaryk University, 3Department of Biochemistry, Faculty of Science,Masaryk University, 4Laboratory of Biomathematics,Czech-BioImaging IPHYS, Institute of Physiology of the Czech Academy of Sciences

Chapters

  • 00:04Introduction
  • 00:52Sample Preparation II
  • 01:47Measurement
  • 05:32Data Analysis
  • 07:52Results: Measurement of the Elastic Moduli of Collagen‐Rich Areas
  • 09:19Conclusion

Summary

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We present a protocol to measure the elastic moduli of collagen-rich areas in normal and diseased liver using atomic force microscopy. The simultaneous use of polarization microscopy provides high spatial precision for localizing collagen-rich areas in the liver sections.

Tags

Liver StiffnessAtomic Force MicroscopyPolarization MicroscopyCollagenFibrogenesisLiver FibrosisParaformaldehydePBSAFMCantileverOptical Calibration

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