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代謝標識および質量分析による老化および非分裂培養細胞におけるタンパク質代謝回転率の測定
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Measurement of Protein Turnover Rates in Senescent and Non-Dividing Cultured Cells with Metabolic Labeling and Mass Spectrometry
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代謝標識および質量分析による老化および非分裂培養細胞におけるタンパク質代謝回転率の測定

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3,092 Views

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08:52 min

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April 06, 2022

DOI:

10.3791/63835-v

DOI:
10.3791/63835-v

•

08:52 min
April 06, 2022

•

11 Views
, ,
1Laboratory of Genetics and Genomics, National Institute on Aging Intramural Research Program,National Institutes of Health, 2Translational Gerontology Branch, National Institute on Aging Intramural Research Program,National Institutes of Health

Summary

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このプロトコルは、パルスSILACによる老化細胞および非分裂細胞の代謝標識、非標的質量分析分析、およびタンパク質半減期の合理化された計算のためのワークフローを記述します。

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Cite this Article

Payea, M., Gorospe, M., Basisty, N. Measurement of Protein Turnover Rates in Senescent and Non-Dividing Cultured Cells with Metabolic Labeling and Mass Spectrometry. J. Vis. Exp. (182), e63835, doi:10.3791/63835 (2022).

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