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Meting van eiwitomloopsnelheden in senescente en niet-delende gekweekte cellen met metabole etikettering en massaspectrometrie
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Measurement of Protein Turnover Rates in Senescent and Non-Dividing Cultured Cells with Metabolic Labeling and Mass Spectrometry
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Meting van eiwitomloopsnelheden in senescente en niet-delende gekweekte cellen met metabole etikettering en massaspectrometrie

Nederlands

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3,092 Views

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08:52 min

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April 06, 2022

DOI:

10.3791/63835-v

DOI:
10.3791/63835-v

•

08:52 min
April 06, 2022

•

11 Views
, ,
1Laboratory of Genetics and Genomics, National Institute on Aging Intramural Research Program,National Institutes of Health, 2Translational Gerontology Branch, National Institute on Aging Intramural Research Program,National Institutes of Health

Summary

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Dit protocol beschrijft de workflow voor metabole etikettering van senescente en niet-delende cellen met gepulste SILAC, ongerichte massaspectrometrie-analyse en een gestroomlijnde berekening van eiwithalfwaardetijden.

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Payea, M., Gorospe, M., Basisty, N. Measurement of Protein Turnover Rates in Senescent and Non-Dividing Cultured Cells with Metabolic Labeling and Mass Spectrometry. J. Vis. Exp. (182), e63835, doi:10.3791/63835 (2022).

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