Measurement of Protein Turnover Rates in Senescent and Non-Dividing Cultured Cells with Metabolic Labeling and Mass Spectrometry

Matthew Payea; Myriam Gorospe; Nathan Basisty

doi:10.3791/63835

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Measurement of Protein Turnover Rates in Senescent and Non-Dividing Cultured Cells with Metabolic Labeling and Mass Spectrometry

JoVE Journal
生化学

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JoVE Journal 生化学

Measurement of Protein Turnover Rates in Senescent and Non-Dividing Cultured Cells with Metabolic Labeling and Mass Spectrometry

3,077 Views

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08:52 min

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April 06, 2022

DOI:

10.3791/63835-v

DOI:

10.3791/63835-v

•

08:52 min

April 06, 2022

•

11 Views

Matthew Payea¹, Myriam Gorospe¹, Nathan Basisty²

¹Laboratory of Genetics and Genomics, National Institute on Aging Intramural Research Program,National Institutes of Health (NIH), ²Translational Gerontology Branch, National Institute on Aging Intramural Research Program,National Institutes of Health (NIH)

概要

This protocol describes the workflow for metabolic labeling of senescent and non-dividing cells with pulsed SILAC, untargeted mass spectrometry analysis, and a streamlined calculation of protein half-lives.

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Measurement of Protein Turnover Rates in Senescent and Non-Dividing Cultured Cells with Metabolic Labeling and Mass Spectrometry

概要

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