Journal
/
생화학
/
Measurement of Protein Turnover Rates in Senescent and Non-Dividing Cultured Cells with Metabolic Labeling and Mass Spectrometry
JoVE 신문
생화학
This content is Free Access.
JoVE 신문 생화학
Measurement of Protein Turnover Rates in Senescent and Non-Dividing Cultured Cells with Metabolic Labeling and Mass Spectrometry

Measurement of Protein Turnover Rates in Senescent and Non-Dividing Cultured Cells with Metabolic Labeling and Mass Spectrometry

3,086 Views

•

08:52 min

•

April 06, 2022

DOI:

10.3791/63835-v

DOI:
10.3791/63835-v

•

08:52 min
April 06, 2022

•

11 Views
, ,
1Laboratory of Genetics and Genomics, National Institute on Aging Intramural Research Program,National Institutes of Health, 2Translational Gerontology Branch, National Institute on Aging Intramural Research Program,National Institutes of Health

Summary

Automatically generated

This protocol describes the workflow for metabolic labeling of senescent and non-dividing cells with pulsed SILAC, untargeted mass spectrometry analysis, and a streamlined calculation of protein half-lives.

Explore More Videos

Protein TurnoverSenescent CellsNon-dividing CellsMetabolic LabelingMass SpectrometrySILACProtein HomeostasisCellular StressAgingNeurodegenerative DiseaseLC-MSTrypsinUreaAmmonium BicarbonateDithiothreitolIodoacetamide

Read Article

Cite this Article

Payea, M., Gorospe, M., Basisty, N. Measurement of Protein Turnover Rates in Senescent and Non-Dividing Cultured Cells with Metabolic Labeling and Mass Spectrometry. J. Vis. Exp. (182), e63835, doi:10.3791/63835 (2022).

Download .ris file

Copy

Share Video

.

Copy